Additional Information:
UFHPL Epic order code: LAB4477
References
- Centers for Disease Control and Prevention (CDC). 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR. 1997 Jan 10; 46:RR-2.
- Giorgi J, Kesson A, Chou CC. Immunodeficiency and infectious diseases. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 25.
- Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.
CPT Code(s):
Specimen Requirements:
Type: Whole blood
- Invert each tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimens so they will arrive at the laboratory within 48 hours of collection. Indicate the date and time of venipuncture on the tubes and on the test requisition.
Sample Volume: Fill the tubes to capacity.
Storage: Store specimens at room temperaturen.
- Ambient: 2 days
- Refrigerated: Unstable
- Frozen: Unstable
- Freeze/Thaw Cycles: Unstable
- Hemolysis
- Specimen refrigerated or frozen
- Clotted specimen
- Contaminated specimen
Use:
- Percentage CD4+
- Absolute CD4+
- Percentage CD8+
- Absolute CD8+
- CD4:CD8 ratio
- CBC with differential
Methodology:
Flow cytometry
Reference Values:
Reference Intervals
95 Percent Confidence Interval |
|||||
---|---|---|---|---|---|
CD |
Age |
Percentage Minimum |
Percentage Maximum |
Cells/mm3 Minumum |
Cells/mm3 Maximum |
Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393. |
|||||
CD4+ |
Neonates |
17 |
52 |
400 |
3500 |
1 week to |
41 |
68 |
1700 |
5300 |
|
2 − 5 |
33 |
58 |
1500 |
5000 |
|
5 − 9 |
33 |
58 |
1400 |
5100 |
|
9 − 15 |
31 |
54 |
1000 |
4600 |
|
15 − 24 |
25 |
50 |
900 |
5500 |
|
2 − 5 |
23 |
48 |
500 |
2400 |
|
5 − 10 |
27 |
53 |
300 |
2000 |
|
10 − 16 |
25 |
48 |
400 |
2100 |
|
Adults |
31 |
59 |
359 |
1519 |
|
CD8+ |
Neonates |
10 |
41 |
200 |
1900 |
1 week to |
9 |
23 |
400 |
1700 |
|
2 − 5 |
11 |
25 |
500 |
1600 |
|
5 − 9 |
13 |
26 |
600 |
2200 |
|
9 − 15 |
12 |
28 |
400 |
2100 |
|
15 − 24 |
11 |
32 |
400 |
2300 |
|
2 − 5 |
14 |
33 |
300 |
1600 |
|
5 − 10 |
19 |
34 |
300 |
1800 |
|
10 − 16 |
9 |
35 |
200 |
1200 |
|
Adults |
12 |
36 |
109 |
897 |
|
T4:T8 ratio |
Adults |
0.92 |
3.72 |
Performed:
Monday - Friday